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Benchmarking of Fluorescence Lifetime Measurement using Time-Frequency Correlated Photons

ORCID
0000-0003-4874-428X
Zugehörigkeit
Fraunhofer Institute for Applied Optics and Precision Engineering
Gäbler, Tobias Bernd;
ORCID
0009-0000-6055-3975
Zugehörigkeit
Fraunhofer Institute for Applied Optics and Precision Engineering
Jain, Nitish;
ORCID
0009-0006-5103-7665
Zugehörigkeit
Leibniz Institute of Photonic Technology
Then, Patrick;
ORCID
0000-0002-3698-5599
Zugehörigkeit
Leibniz Institute of Photonic Technology
Eggeling, Christian;
ORCID
0000-0001-8361-892X
Zugehörigkeit
Technical University of Darmstadt
Gräfe, Markus;
ORCID
0000-0001-8723-9986
Zugehörigkeit
Fraunhofer Institute for Applied Optics and Precision Engineering
Gili, Valerio Flavio

The investigation of fluorescence lifetime became an important tool in biology and medical science. So far, establish methods of fluorescence lifetime measurements require the illumination of the investigated probes with pulsed or amplitude-modulated light. In this paper, we examine the limitations of an innovative method of fluorescence lifetime using time-frequency correlated photons generated by a continuous-wave source. For this purpose, we investigate the lifetime of IR-140 to demonstrate the functional principle and its dependencies on different experimental parameters.

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