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Benchmarking of Fluorescence Lifetime Measurement using Time-Frequency Correlated Photons

ORCID
0000-0003-4874-428X
Affiliation
Fraunhofer Institute for Applied Optics and Precision Engineering
https://ror.org/02afjh072
https://ror.org/05qpz1x62
Gäbler, Tobias Bernd;
ORCID
0009-0000-6055-3975
Affiliation
Fraunhofer Institute for Applied Optics and Precision Engineering
https://ror.org/02afjh072
Jain, Nitish;
ORCID
0009-0006-5103-7665
Affiliation
Leibniz Institute of Photonic Technology
https://ror.org/02se0t636
https://ror.org/05qpz1x62
Then, Patrick;
ORCID
0000-0002-3698-5599
Affiliation
Leibniz Institute of Photonic Technology
https://ror.org/02se0t636
https://ror.org/05qpz1x62
Eggeling, Christian;
ORCID
0000-0001-8361-892X
Affiliation
Technical University of Darmstadt
https://ror.org/05n911h24
Gräfe, Markus;
ORCID
0000-0001-8723-9986
Affiliation
Fraunhofer Institute for Applied Optics and Precision Engineering
https://ror.org/02afjh072
Gili, Valerio Flavio

The investigation of fluorescence lifetime became an important tool in biology and medical science. So far, establish methods of fluorescence lifetime measurements require the illumination of the investigated probes with pulsed or amplitude-modulated light. In this paper, we examine the limitations of an innovative method of fluorescence lifetime using time-frequency correlated photons generated by a continuous-wave source. For this purpose, we investigate the lifetime of IR-140 to demonstrate the functional principle and its dependencies on different experimental parameters.

This data set offers all program codes, raw data and plots used for the related publication.

Category

published:
2025
DOI:
10.5281/zenodo.14654235
Language:
English
Keywords:
FluoreszenzmikroskopGND; QuantentechnologieGND
Format:
.zip (Zip Archive)
.pdf (Adobe Portable Document Format)
.txt (Text File)
.csv (Comma-Seperated Values)
.xlsx (Microsoft Office - OOXML - Spreadsheet)
.svg (Scalable Vector Graphics (SVG))
.png (Portable Network Graphics (PNG))
other Format
SDNB:
530 Physics
Institution:
Institut für Angewandte Physik
Access permissions:
External access
is supplement to:
Benchmarking of Fluorescence Lifetime Measurement using Time-Frequency Correlated Photons(Preprint)
DOI: 10.48550/arXiv.2501.08870
is supplement to:
Benchmarking of Fluorescence Lifetime Measurement using Time-Frequency Correlated Photons(Peer Review)
DOI:
requires:
quTAG python code(Software)

Funding

Name of Funder:
Bundesministerium für Bildung und Forschung (BMBF)
Funder ID:
https://ror.org/04pz7b180
Grant Title:
Zweiphotonenabsorption zur Anwendung für quanten-gestützte Fluoreszenzmikroskopie (LIVE2QMIC)
Grant Number:
13N15954
Grant URI:
https://www.quantentechnologien.de/forschung/foerderung/enabling-technologies-fuer-die-quantentechnologien/live2qmic.html
Name of Funder:
Bundesministerium für Bildung und Forschung (BMBF)
Funder ID:
https://ror.org/04pz7b180
Grant Title:
DNA-stabilisierte Metallquantencluster als Emitter verschränkter Photonen (QC4EP)
Grant Number:
13N16758
Grant URI:
https://www.quantentechnologien.de/forschung/foerderung/wissenschaftliche-vorprojekte-wivopro-photonik-und-quantentechnologien/qc4ep.html
Name of Funder:
Bundesministerium für Bildung und Forschung (BMBF)
Funder ID:
https://ror.org/04pz7b180
Grant Title:
Photonics Research Germany
Grant Number:
13N15713
Name of Funder:
Bundesministerium für Bildung und Forschung (BMBF)
Funder ID:
https://ror.org/04pz7b180
Grant Title:
Photonics Research Germany
Grant Number:
13N15717
Name of Funder:
European Union
Funder ID:
https://ror.org/019w4f821
Grant Title:
Qu-Test
Grant Number:
101113901
Grant URI:
https://doi.org/10.3030/101113901
Name of Funder:
Deutsche Forschungsgemeinschaft (DFG)
Funder ID:
https://ror.org/018mejw64
Grant Title:
EXC 2051: Balance of the Microverse
Grant Number:
390713860
Grant URI:
https://gepris.dfg.de/gepris/projekt/390713860
Name of Funder:
Deutsche Forschungsgemeinschaft (DFG)
Funder ID:
https://ror.org/018mejw64
Grant Title:
Multiphoton laser scanning microscope with FLIM module
Grant Number:
460889961
Grant URI:
https://gepris.dfg.de/gepris/projekt/460889961

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